Sequence Analysis of F Gene of SF02 Isolate of Goose Paramyxovirus and Its Identification by Multiplex RT-PCR

ZOU Jian, SHAN Song-Hua¹, YAO Long-Tao², GONG Zu-Xun^*
( Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China; ¹Shanghai Entry-Exit Inspection and Quarantine Bureau of PRC, Shanghai 200135, China; ²Fengxian Veterinary Station of Shanghai, Shanghai 201400, China )

Abstract    The complete F gene of SF02 of goose paramyxovirus (GPV) has been cloned and analyzed. The sequence analysis demonstrated that the F gene of SF02 contains 1 662 nt and encodes 553 amino acids, and its cleavage activation site of F gene has the same deduced amino acid sequence, ¹¹²R-R-Q-K-R-F¹¹⁷, as the velogenic (highly pathogenic) strain of newcastle disease virus. The latter correlated with the virulence of the isolate in biological assays. The F gene of SF02 isolate with the domestic standard velogenic strain NDV, F48E9, shared 86.5% homology in nucleotide and 90.8% homology in amino acid sequences. The SF02 isolate is closer to some NDV strains prevalent in Taiwan and West-European countries in recent years. Based on the F gene sequence a multiplex RT-PCR method has been developed. It could be used for the discrimination of GPV from NDV.
Key words    GPV; NDV; F gene; sequence analysis; multiplex RT-PCR

^*Corresponding author: Tel, 86-21-64374430-5220, Fax 86-21-64746510; e-mail, [email protected]